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Document Type |
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Thesis |
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Document Title |
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THE EFFECT OF DATE FLAVONOIDS ON ANGIOGENESIS IN HEPG2 CELLS AS A TARGET OF CHEMOTHERAPY تأثير فلافونيدات التمر على تكوُّن الأوعية الدموية في خلايا الكبد جي 2 المستهدفة للعلاج الكيميائي |
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Subject |
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Faculty of Science |
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Document Language |
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Arabic |
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Abstract |
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Hepatocellular carcinoma (HCC) is ranked as the second cancer cause of death worldwide characterized as a highly aggressive and angiogenic-dependent tumor. Indeed, angiogenesis is critical for solid tumors like HCC to develop and metastases. Sukkari is a famous date cultivar grown in Saudi Arabia. Similar to other date extracts, Sukkari date extract (SDE) contains flavonoids among other compounds that exert biological activity. The present study aimed to evaluate the anti-angiogenic effect of SDE on HCC development. The study was carried out on the HepG2 cell line. Firstly, the anti-proliferative effect of different SDE concentrations (20,30,40,50 and 60 mg/ml) were assessed using WST-1 and crystal violate dye. Then, the cell death level was elucidated by measuring the apoptotic effect of 20 and 30 mg/ml SDE using flow cytometry. The effect of different SDE concentrations on migration rate by wound healing assay. Finally, the real-time polymerase chain reaction (qt-PCR) was conducted to estimate the relative gene expression of specific angiogenic genes VEGF and VEGFR2 by using the IC50 of SDE. Results have shown that SDE significantly reduces HepG2 viability in a concentration-dependent and time-dependent manner. Despite this, treating the cells with low SDE concentrations had been found to induce HepG2 apoptosis. Likewise, high SDE concentrations were inhibiting the migration of the cells. Significantly, the IC50 (47 mg/ml) concentration of SDE was capable to inhibit mRNA expression of VEGFA/VEGFR2 in HepG2. The results concluded that SDE is susceptible to inhibiting HCC angiogenesis as well as cellular proliferation in HepG2.
Keywords: Hepatocellular carcinoma, Angiogenesis, Sukkari date extract, Flavonoids, Vascular endothelial growth factor |
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Supervisor |
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Prof. Dr. Khaled Omar Abu annja |
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Thesis Type |
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Master Thesis |
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Publishing Year |
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1445 AH
2023 AD |
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Co-Supervisor |
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Dr. Nouf Al-Shareef |
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Added Date |
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Wednesday, December 27, 2023 |
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Researchers
| مروج جابر ابوعايد | Ayeid, Morooj Jaber | Researcher | Master | |
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