|
Document Type |
: |
Thesis |
|
Document Title |
: |
Cordycepin attenuates testosterone-induced benign prostate hyperplasia in rats through inhibition of oxidative stress and inflammation كورديسيبن يقلل من تضخم البروستات الحميد المحدث بالتيستوستيرون عن طريق تثبيط الأكسدة والالتهابات |
|
Subject |
: |
faculty of Pharmacy |
|
Document Language |
: |
Arabic |
|
Abstract |
: |
Benign prostatic hyperplasia (BPH) is a disease that commonly affects elderly men. Cordycepin is an adenosine analog with a wide range of pharmacological activities including antiproliferative and prostatic smooth muscle relaxant effects. This study was designed to assess the actions of cordycepin in testosterone-induced BPH in rats. Animals were divided into six treatment groups: control, cordycepin-alone (10 mg/kg), testosterone-alone (3 mg/kg), cordycepin (5 mg/kg) + testosterone, cordycepin (10 mg/kg) + testosterone, and finasteride (0.5 mg/kg) + testosterone. Treatments were continued daily, 5 days a week, for 4 weeks. Cordycepin significantly prevented the increase in prostate weight and prostate index induced by testosterone. This was confirmed by histopathological examinations. Cordycepin’s anti-proliferative activity was further defined by its ability to inhibit cyclin-D1 and proliferating cell nuclear antigen (PCNA) expression. In addition, cordycepin exhibited significant antioxidant properties as proven by the prevention of lipid peroxidation, reduced glutathione diminution, and superoxide dismutase exhaustion. This was paralleled by anti-inflammatory activity as shown by the inhibition of interleukin-6, tumor necrosis factor-α, and nuclear factor-κB expression in prostatic tissues. It also enhanced apoptosis as demonstrated by its ability to enhance and inhibit mRNA expression of Bax and Bcl2, respectively. Western blot analysis indicated that cordycepin augmented phospho-AMP-activated protein kinase (p-AMPK) and inhibited p-AKT expression. Collectively, cordycepin has the ability to prevent testosterone-induced BPH in rats. This is mediated, at least partially, by its antiproliferative, antioxidant, anti-inflammatory, and pro-apoptotic actions in addition to its modulation of AMPK and AKT activation.
Keywords: cordycepin; benign prostatic hyperplasia; testosterone; AMPK; AKT |
|
Supervisor |
: |
Prof. Ashraf B. Abdel-Naim |
|
Thesis Type |
: |
Master Thesis |
|
Publishing Year |
: |
1445 AH
2023 AD |
|
Added Date |
: |
Sunday, November 26, 2023 |
|
Researchers
| سامي أحمد العيسي | Alessi, Sami Ahmed | Researcher | Master | |
|